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KMID : 1143620190230010035
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Korean Journal of Nuclear Medicine Technology
2019 Volume.23 No. 1 p.35 ~ p.39
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Analysis of HBeAg and HBV DNA Detection in Hepatitis B Patients Treated with Antiviral Therapy
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Chun Jun-Hong
Chae Hong-Ju
Park Mi-Sun
Lim Soo-Yeon
Yoon Seon-Hee
Lee Sun-Ho
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Abstract
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Purpose: Hepatitis B virus (hepatitis B virus, HBV) infection is a worldwide major public health problem and it is known as a major cause of chronic hepatitis, liver cirrhosis and liver cancer. And serologic tests of hepatitis B virus is essential for diagnosing and treating these diseases. In addition, with the development of molecular diagnostics, the detection of HBV DNA in serum diagnoses HBV infection and is recognized as an important indicator for the antiviral agent treatment response assessment. We performed HBeAg assay using Immunoradiometric assay (IRMA) and Chemiluminescent Microparticle Immunoassay (CMIA) in hepatitis B patients treated with antiviral agents. The detection rate of HBV DNA in serum was measured and compared by RT-PCR (Real Time - Polymerase Chain Reaction) method
Materials and Methods : HBeAg serum examination and HBV DNA quantification test were conducted on 270 hepatitis B patients undergoing anti-virus treatment after diagnosis of hepatitis B virus infection. Two serologic tests (IRMA, CMIA) with different detection principles were applied for the HBeAg serum test. Serum HBV DNA was quantitatively measured by real-time polymerase chain reaction (RT-PCR) using the Abbott m2000 System.
Results: The detection rate of HBeAg was 24.1% (65/270) for IRMA and 82.2% (222/270) for CMIA. Detection rate of serum HBV DNA by real-time RT-PCR is 29.3% (79/270). The measured amount of serum HBV DNA concentration is 4.8×107 ± 1.9×108 IU/mL(mean ± SD). The minimum value is 16 IU/mL, the maximum value is 1.0×109 IU/mL, and the reference value for quantitative detection limit is 15 IU/mL. The detection rates and concentrations of HBV DNA by group according to the results of HBeAg serological (IRMA, CMIA)tests were as follows.
1) Group Ⅰ(IRMA negative, CMIA positive, N = 169), HBV DNA detection rate of 17.7% (30/169), 6.8×105 ± 1.9×106 IU/mL
2) Group Ⅱ (IRMA positive, CMIA positive, N = 53), HBV DNA detection rate 62.3% (33/53), 1.1×108 ±2.8×108 IU/mL
3) Group Ⅲ (IRMA negative, CMIA negative, N = 36), HBV DNA detection rate 36.1% (13/36), 3.0×105 ±1.1×106 IU/mL
4) Group Ⅳ(IRMA positive, CMIA negative, N = 12), HBV DNA detection rate 25% (3/12), 1.3×103 ± 1.1×103 IU/mL
Conclusion: HBeAg detection rate according to the serological test showed a large difference. This difference is considered for a number of reasons such as characteristics of the Ab used for assay kit and epitope, HBV of genotype.
Detection rate and the concentration of the group-specific HBV DNA classified serologic results confirmed the high detection rate and the concentration in Group Ⅱ(IRMA-positive, CMIA positive, N = 53).
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KEYWORD
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HBV(hepatitis B virus), IRMA, CMIA, HBV DNA
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